KS supp

KS dropout

Sherman G&F supp

Styles G&F supp

Styles G&F dropout

CSHL 1997 supp

CSHL 1997 dropout

CSHL 1997 HC dropout+supp

Stanford Biochem dropout

 

mg/l

mg/l

mg/l

mg/l

mg/l

mg/l

mg/l

mg/L

mg/l

adenine sulfate

50

18

20

55

87

20

19

20

20

uracil

20

18

20

22

87

20

76

35

20

tryptophan

50

71

20

80

87

20

76

80

20

histidine-HCl

20

18

20

63

87

20

76

20

20

arginine-HCl

20

18

20

 

87

20

76

20

20

methionine

20

18

20

 

87

20

76

20

20

tyrosine

30

53

30

 

87

30

76

60

30

leucine

100

71

30

219

173

100

380

80

30

isoleucine

30

71

30

 

87

30

76

80

 

lysine-HCl

100

53

30

183

87

30

76

120

30

phenylalanine

50

44

50

 

87

50

76

50

50

glutamic acid

100

89

100

 

87

100

76

100

 

aspartic acid

100

89

100

 

87

100

76

100

 

valine

150

133

150

 

87

150

76

150

 

threonine

200

177

200

 

87

200

76

200

200

serine

400

354

400

 

87

400

76

400

 

 

 

 

 

 

 

 

 

 

 

inositol

 

 

 

60

36

 

76

 

 

alanine

 

35

 

 

87

 

76

 

 

asparagine

 

 

 

 

87

 

76

 

 

cysteine

 

35

 

 

87

 

76

 

 

glutamine

 

 

 

 

87

 

76

 

 

glycine

 

 

 

 

87

 

76

 

 

proline

 

35

 

 

87

 

76

 

 

PABA

 

 

 

 

9

 

8

 

 

 

Sources:

Katja Schwartz/Botstein lab standard supplements for liquid media and dropout plates

Fred Sherman's Guthrie and Fink article

Cora Styles's Guthrie and Fink article

Cold Spring Harbor yeast course manual 1997: supplements, dropout plates, and Hartwell Complete

Stanford Biochemistry Department media facility